Plant regeneration of Withania somnifera.

Author: Zhengwei LUO ¹ ; Yiming SUN ; Cuiping LV ; Fengying WANG ; Jiwei SUN ; Min SUN
Author Information

1. SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China.
Publication Type:Journal Article
MeSH: Culture Media; pharmacology; Plant Growth Regulators; pharmacology; Regeneration; drug effects; Tissue Culture Techniques; Withania; drug effects; growth & development
From: China Journal of Chinese Materia Medica 2012;37(7):897-900
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study tissue culture and plant regeneration of Withania somnifera.
METHODLeaves of W. somnifera were used for explants, effects of different plant growth substances on callus and shoot induction were studied, different medium and plant growth substances for rooting induction was optimized.
RESULT AND CONCLUSIONThe best plant growth substances combination for callus induction was MS + 1.0 mg x L(-1) 2,4-D + 0.1 mg x L(-1) KT. The optimal medium for germination was MS + 1.0 mg x L(-1) 6-BA + 0.1 mg x L(-1) NAA. The best medium and plant growth substances combination for rooting induction was 1/2MS + 0.5 mg x L(-1) NAA, transplant survival rate of plantlet reached 92% in humus soil-pearlite (1:1).