Simultaneous determination of five active components in resina draconis and its extract by HPLC.
- Author:
Yun LI
1
;
Wei XIAO
;
Jianping QIN
;
Ying GUO
;
Qianqian ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Chalcones; analysis; Chromatography, High Pressure Liquid; methods; Dracaena; chemistry; Flavones; analysis; Resins, Plant; analysis; Stilbenes; analysis
- From: China Journal of Chinese Materia Medica 2012;37(7):929-933
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for simultaneous determination of contents of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis and its extracts.
METHODKromasil 100-5C18 column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL x min(-1) and the column temperature at 40 degrees C. The detective wave length of loureirin A and B was detected at 278 nm, and 7,4'-dihydroxy flavone, pterostilbene and resveratrol was at 319 nm.
RESULTAll the five active components reached the resolved peaks within 40 min, indicating a good linearity (r > or = 0.999 7). The average recoveries of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis were 102.9%, 96.81%, 97.29%, 100.7% and 103.7%, with RSDs of 0.23%, 1.5%, 0.42%, 0.58% and 0.34%, respectively. The average recoveries of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in extract of resina draconis were 102. 2% , 96. 93%, 97. 90% , 102.0% and 103.3%, with RSDs of 1.7%, 0.91%, 1.4%, 1.5% and 1.2%, respectively.
CONCLUSIONThe method is so easy, accurate, highly repeatable and stable that it provides good reference for the quality control of resina draconis and its extracts.