Increased expression of transient receptor potential melastatin 7 in mouse cardiac fibroblasts post myocardial infarction
10.3321/j.issn:0253-3758.2008.07.015
- VernacularTitle:瞬时受体电位亚族M7在小鼠心肌梗死后的表达变化及其意义
- Author:
Ming-Jiang LI
1
;
Yong-Ming ZHOU
;
Yan-Hong TANG
;
Feng CAO
Author Information
1. 武汉大学人民医院
- Keywords:
Myocardial infarction;
Fibroblasts;
Fibrosis
- From:
Chinese Journal of Cardiology
2008;36(7):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe transient receptor potential melastatin 7-like (TRPMTL) expression changes post myocardial infarction (MI) in mouse cardiac fibroblast (CF).Methods TRPM7 expression and Ca2+ influx in CF from MI and control mice were quantified by mRNA RT-PCR and whole cell patch clamp technique.Results (1) TRPM7 expression was significantly upregulated post MI and Ca2+ influx of CF were significantly increased post MI [ (7.4 ± 0.7 ) pA/pF vs.( 16.2 ± 1.7) pA/pF, P < 0.01 ] and Ca2+ influx of CF increased 3-fold under lower pH condition; (2) These effects could be blocked by knock-out TRPM7 gene with SiRNA.conclusion TRPM7L upregulation post MI and under lower pH condition are responsible for increased Ca2+ influx in CF.
