Human bone marrow mesenchymal stem cells cocultured with semi-permeable membrane separated neonatal rat ventricular myocytes differentiated into cardiomyocyte phenotype
10.3321/j.issn:0253-3758.2008.07.017
- VernacularTitle:微环境中人骨髓间充质干细胞向心肌细胞表型分化的实验研究
- Author:
Xiao-Hong LI
1
;
Xi-Yong YU
;
Qiu-Xiong LIN
;
Zhi-Xin SHAN
;
Guang-Feng ZHANG
;
Xiao ZHANG
;
Chun-Yu DENG
;
Yong-Heng FU
;
Su-Juan KUANG
;
Wei HUANG
;
Min YANG
;
Shu-Guang LIN
Author Information
1. 广东省人民医院
- Keywords:
Myocytes,cardiac;
Mesenchymal stem cells;
Cell differentiation;
Microenvironment
- From:
Chinese Journal of Cardiology
2008;36(7):651-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ability of human bone marrow mesenchymal stem cells (hBMSCs), eocultured with semi-permeable membrane separated neonatal rat ventricular myocytes, to differentiate into cardiomyocytes.Methods hBMSCs were isolated and purified by density gradient centrifugation and adherence screening method.cells were expanded as undifferentiated cells in culture for more than 3 passages and their phenotypes were identified with flow cytometer, hBMSCs were cocuhured with neonatal rat ventricular myocytes in a rate of 1:10 separated by semi-permeable membrane.GATA4 mRNA was detected by RT-PCR; Immunocytochemistry, and Immunostaining were used to detect sarcomeric α-actinin, desmin, cTnT, and cTnI protein level.Results CD29 (98.64% ± 0.80%) and CD44 (96.70% ± 1.50% ) were the major surface markers of hBMSCs.After coculturing with semi-permeable membrane separated neonatal rat ventricular myocytes, the first contraction of single cells was noted at day 7 and GATA4 expression was detected on these cells by RT-PCR after 1 to 3 weeks coculture.Desmin, sacomefic ±-actinin, cTnI and cTnT could be detected by immunocytochemistry and immunostaining on some of these cells.conclusion hBMSCs possess the potential to differentiate into myocardial cell phenotype in the cardiac microenvironment.Direct contact with cardiomyocytes was not necessary required for hBMSCs differentiation.
