Association among circulating endothelial progenitor cells, insulin resistance and severity of coronary lesions in patients with coronary artery disease
10.3321/j.issn:0253-3758.2008.08.010
- VernacularTitle:冠心病患者胰岛素水平与内皮祖细胞及冠状动脉病变的相关性
- Author:
De-Hui QIAN
1
;
Lan HUANG
;
Xiao-Hui ZHAO
;
Yin-Pin ZHOU
;
Bin CUI
;
Yao-Ming SONG
;
Ai-Min LI
;
Xiao-Lan FU
Author Information
1. 第三军医大学新桥医院
- Keywords:
Coronary disease;
Insulin resistance;
Stem cells
- From:
Chinese Journal of Cardiology
2008;36(8):714-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the number and activity of circulating endothelial progenitor cells (EPCs), insulin resistance and severity of coronary lesions in patients with coronary artery disease (CAD). Methods Patients with coronary angiography evidenced CAD were divided in insulin resistance group ( IR, n = 25 ) and insulin sensitive group ( IS, n = 44) according to insulin level, 25 health volunteers served as control. Circulating EPCs were marked as KDR/CD133<'+ cells via fluorescence- activated cell sorter analysis. EPCs were also isolated from peripheral blood and cultured in vitro for 7 days, identified by DiI-acLDL uptake and lectin staining methods. EPCs migration activities were determined by modified Boyden chamber assay, EPCs proliferation activities were determined by MTT assay. Result Circulating EPCs number was significantly lower in IR group compared with IS group [ (0. 34±0. 08 ) ‰ vs. (0. 47±0. 09 )‰, P < 0. 01 ] and control group ( P < 0. 05 ). Both insulin resistence index (r = - 0. 291, P = 0. 01) and Gensini score ( r = - 0. 3984, P = 0. 006) were negatively correlated with number of circulating EPCs. Proliferation and migration capacities of EPCs were also significantly lower in IR group compared to those in IS group ( all P < 0. 05 ) and control group ( all P < 0. 05). Conclusions Insulin resistence/hyperinsulinemia could aggravate severity of coronary artery lesions via reducing the number and activities of circulating EPCs in patients with CAD.
