Disturbed shear stress affects disposition of caveolin-1 in endothelial cells
10.3321/j.issn:0253-3758.2008.10.016
- VernacularTitle:扰动剪切流场对血管内皮细胞小窝蛋白再分布和表达量的影响
- Author:
Bing LIU
1
;
Shao-Xi CAI
;
Li ZHANG
Author Information
1. 重庆大学
- Keywords:
Disturbed shear stress;
Cavcelin-1;
Endothelial cell;
Read time-PCR
- From:
Chinese Journal of Cardiology
2008;36(10):927-930
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the distribution and expression changes of cavcelin-1 under shear stress in a turbulence flow channel model mimicking susceptible sites of atheroscleresis in vitro.Methods Human umbilical vein endothelial cells (HUVECs) were inoculated to flow channel and then exposed to distubed shear stress for6,12,24 and 48 hours,respectively.HUVECs inoculated to flow channel without shear stress served as controls.Immunofluorescence staining was performed with SABC-FITC to detect the caveolin-1 distribution and RT-PCR was used to determine the mRNA expression of caveolin-1 gone in these cells.Results Confocal microcopy revealed ellipse-like shaped HUVECs under disturbed shear stress and caveulin-1 didn't move toward to the upstream edge of HUVECs but moved toward to the edge of HUVECs,and expression of caveolin-1 gone in HUVECs was significantly downregulated after exposure to shear stress for 48 hours compared to HUVECs without shear stress stimulation.Conclusion Disturbed shear stress affected disposition and expression of caveolin-1 which might be responsible for shear stress induced endothelial cell pathological atherosclerotic changes.
