Time course of ubiqultIn and rat component 3 of proteasome expressions and associnfion with common carotid artery intimal proliferation after balloon injury in rats
10.3321/j.issn:0253-3758.2008.10.017
- VernacularTitle:泛素及大鼠蛋白酶体第三亚基在大鼠颈总动脉球囊损伤后的表达
- Author:
Xin-Jing CHEN
1
;
Xiao-Dong PU
Author Information
1. 福建医科大学省立临床学院
- Keywords:
Tunica intima;
Ubiquitin;
Hyperplasin;
Carotid artery injury;
Rat
- From:
Chinese Journal of Cardiology
2008;36(10):931-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic ubiquitin and rat component 3 of proteasome (RC3) change and association with intimal proliferation in the common carotid artery of rats after balloon injury.Methods Male SD rats were randomly divided into two groups:sham operation group (n=8),ballon injury group at various time points (1,4,7,14,21,28-days post injury,n=8each).The balloon injury model was established by deendothelializing the rat common carotid aortic artery by inflated 2F balloon catheter.HE staining was used to observe the ratio of intima-media (I/M) thickness of the injured arteries under optical microscopy.The expression levels of ubiquitin and RC3 mRNA were detected with RT-PCR.The expression level of ubiquitin protein was measured by immunohistochemistry method.Results The maximal intimal proliferation and peak intima-media ratio (2.31±0.43 vs.control 0.02 ±0.005,P＜0.01) were seen at 28th day post injury.The peak expression of ubiquitin was observed at the 7th day post injury (1.33±0.26 vs.control 0.21 ±0.04,P＜0.01) and peak RC3 expression was found at the 14th day post injury (1.35 ±0.26 vs.control 0.31± 0.06,P＜0.01).The protein expression of ubiquitin was also seen at the 14th day post injury (21.53±4.09 vs.control 4.21±0.78,P＜0.01).The expression of ubiquitin protein was positively correlated with intimal proliferation (r=0.827,P＜0.05).Conclusion The upregulated ubiquitin and RC3 expressions after balloon injury might play an important role in intimal proliferation in this model.
