Nuclear receptor Nur77 inhibits oxidized low density lipoprotein induced lipid loading in macrophages.
- Author:
Liu-hua HU
1
;
Ben HE
;
Ling-hong SHEN
;
Lei ZHOU
;
Jun PU
;
Li-sheng JIANG
;
Qin SHAO
;
Li WANG
;
Jin-Zhang ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD36 Antigens; metabolism; Cell Line; Cholesterol; metabolism; DNA, Complementary; DNA-Binding Proteins; genetics; Lipid Metabolism; Lipoproteins, LDL; metabolism; Macrophages; metabolism; Mice; Nuclear Receptor Subfamily 4, Group A, Member 1; Receptors, Steroid; genetics; Transfection
- From: Chinese Journal of Cardiology 2008;36(11):1032-1036
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Nur77 on lipid loading in macrophages exposed to 40 microg/ml oxidized low density lipoprotein (ox-LDL).
METHODSStable RAW264.7 strain expressing green fluorescent protein (GFP) or GFP-Nur77 was established by G418 screening after transfection with corresponding plasmids and identified by Western blot. After 24 h stimulation with ox-LDL, intracellular lipid loading of each strain was observed by Oil Red O dyeing, and the intracellular cholesterol level was measured by liquid chromatographic-mass spectrometry (LC-MS). The transcriptional changes of CD36 and ABCA1 were monitored by Real Time Quantitative-PCR, while the expressions of these two proteins were assayed by flow cytometry and Western blot, respectively.
RESULTSAfter 24 h stimulation with ox-LDL, intracellular total cholesterol and esterified cholesterol concentration in GFP-Nur77-RAW264.7 were significantly dropped by 26.15% and 30.93% respectively (P < 0.05 vs. GFP-RAW264.7). The transcription and expression of ABCA1 in GFP-Nur77-RAW264.7 were significantly increased while the transcription and expression of CD36 were significantly reduced (all P < 0.05 vs. GFP-RAW264.7).
CONCLUSIONOrphan nuclear receptor Nur77 reduced ox-LDL induced intracellular lipid loading in macrophages by inhibiting lipid influx and enhancing lipid efflux.
