Nuclear receptor Nur77 inhibits oxidized low density lipoprotein induced lipid loading in macrophages
10.3321/j.issn:0253-3758.2008.11.017
- VernacularTitle:孤儿核受体Nur77抑制小鼠巨噬细胞脂质沉积
- Author:
Liu-Hua HU
1
;
Ben HE
;
Ling-Hong SHEN
;
Lei ZHOU
;
Jun PU
;
Li-Sheng JIANG
;
Qin SHAO
;
Li WANG
;
Jin-Zhang ZENG
Author Information
1. 上海交通大学医学院附属仁济医院
- Keywords:
Atherosclerosis;
Macrophnges;
Antigen,CD36
- From:
Chinese Journal of Cardiology
2008;36(11):1032-1036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Nur77 on lipid loading in macrophages exposed to 40 μg/ml oxidized low density iipoprotein (ox-LDL).Methods Stable RAW264.7 strain expressing green fluorescent protein (GFP) or GFP-Nur77 was established by G418 screening after transfection with corresponding plasmids and identified by Western blot.After 24 h stimulation with ox-LDL.intracellular lipid loading of each strain was observed by Oil Red O dyeing.and the intracellular cholesterol level was measured by liquid chromatographic-mass spectrometry (LC-MS).The transcriptional changes of CD36 and ABCA1 were monitored by Real Time Quantitative-PCR,while the expressions of these two proteins were assayed by flow cytometry and Western blot,respectively.Results After 24 h stimulation with ox-LDL,intracellular total cholesterol and esterified cholesterol concentration in GFP-Nur77-RAW264.7 were significantly dropped by 26.15% and 30.93% restxectively (P＜0.05 vs.GFP-RAW264.7).The transcription and expression of ABCA1 in GFP-Nur77-RAW264.7 were significantly increased while the transcription and expression of CD36 were significantly reduced (all P＜0.05 vs.GFP-RAW264.7).Conclusion Orphan nuclear receptor Nur77 reduced ox-LDL induced intracellular lipid loading in macrophages by inhibiting lipi d influx and enhancing lipid efflux.
