Determination of tolerance ability of platelet to the change of solution osmotic pressure and its significance.
- Author:
Xi-Lin OUYANG
1
;
Jing-Han LIU
;
Dayong GAO
Author Information
1. Department of Blood Transfusion, The general Hospital of PLA, Centre for Clinic Blood Transfusion Medicine of PLA, Beijing 100853, China. ouyang70@plagh.com.cn
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
drug effects;
metabolism;
Humans;
Hypotonic Solutions;
pharmacology;
Isotonic Solutions;
pharmacology;
Osmotic Pressure;
P-Selectin;
biosynthesis;
Phosphatidylserines;
biosynthesis;
Saline Solution, Hypertonic;
pharmacology;
Sodium Chloride;
pharmacology
- From:
Journal of Experimental Hematology
2003;11(1):89-91
- CountryChina
- Language:Chinese
-
Abstract:
In order to determine the tolerance ability of platelet to change of osmotic pressure in solution, the isotonic fresh platelets were exposed to a series of crystal salt solutions with osmotic pressure range from 47 to 611 mOsm for 15 minutes. Then the platelets were returned to isotonic condition and kept for 15 minutes. The expressions of phosphatidylserine and CD62p were assayed in platelets. The results showed that the phosphatidylserine and CD62p expressions were increased when the osmotic pressure of solution was below 238 mOsm, but no significant rise was detected when the platelets were exposed to 611 mOsm solution. No increases of positive rate of CD62p and phosphatidylserine were detected in platelets returned to isotonic condition. It is concluded that platelets are sensitive to hypoosmotic solution and tolerated to hyperosmotic solution. Exceeding the platelet safe volume limitation may lead to injure of platelet osmosis in crystal salt solution.
