Human bone marrow mesenchymal stem cells express multiple hematopoietic growth factors.
- Author:
Guang-Rong ZHU
1
;
Xiao-Yu ZHOU
;
Hua LU
;
Jian-Wei ZHOU
;
Ai-Ping LI
;
Wei XU
;
Jian-Yong LI
;
Cheng-Ya WANG
Author Information
1. Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
metabolism;
Cell Differentiation;
drug effects;
Gene Expression Regulation;
drug effects;
Granulocyte Colony-Stimulating Factor;
genetics;
Granulocyte-Macrophage Colony-Stimulating Factor;
genetics;
Hematopoietic Cell Growth Factors;
genetics;
Humans;
Hydrocortisone;
pharmacology;
Mesenchymal Stromal Cells;
metabolism;
RNA, Messenger;
analysis;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Experimental Hematology
2003;11(2):115-119
- CountryChina
- Language:Chinese
-
Abstract:
To study the biological role of human cultured bone marrow mesenchymal stem cell (BM-MSC) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors, RT-PCR was used to analyze the expression of SCF, Flt3-ligand, TPO, LIF, G-CSF, GM-CSF, IL-3, IL-6 and IL-11 at mRNA level for human BM-MSC from healthy donors and patients with leukemia and lymphoma. BM-MSC were incubated with or without hydrocortison (HC). The results clearly showed that the cultured BM-MSC expressed mRNA of SCF, Flt3-ligand, TPO, LIF, IL-6 and IL-11 at passages 3 up to 15, but did not express G-CSF, GM-CSF and IL-3. The same expression pattern of above cytokines was seen also for the patient's BM-MSC. HC was able to induce BM-MSC to express G-CSF but not to express GM-CSF. BM-MSC seemed not to change morphologically after incubation with HC for up to 21 days. In conclusion, both normal and patient BM-MSC should be potential to promote hematopoiesis according to their expression of multiple hematopoietic cytokines, and HC is able to induce hematopoietic growth factor expression.
