Proliferation and differentiation of human CD34+ hematopoietic stem/progenitor cells induced by Panax notoginosides.
- Author:
Xu-Dai QIAN
1
;
Rui-Lan GAO
;
Ke MA
;
Jin-Mei JIN
Author Information
1. Department of Hematology, Affiliated Hospital of Zhejiang College of Traditional Chinese Medicine, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
analysis;
Antigens, CD34;
analysis;
Antigens, Differentiation, Myelomonocytic;
analysis;
Cell Differentiation;
drug effects;
Cell Division;
drug effects;
Ginsenosides;
pharmacology;
Hematopoietic Stem Cells;
cytology;
drug effects;
Humans;
Lewis X Antigen;
analysis;
Sialic Acid Binding Ig-like Lectin 3
- From:
Journal of Experimental Hematology
2003;11(2):120-123
- CountryChina
- Language:Chinese
-
Abstract:
The object of this study was to explore the effects of Panax notoginosides (PNS) on proliferation and differentiation of human CD34(+) stem/progenitor cells. CD34(+) cells were isolated from human bone marrow by using immune beads of Dynal M- 450 system. The cells were exposed to PNS at different concentrations in both liquid and semi-solid culture for 14 days. The cells were marked with monoclonal antibodies and analyzed by flow cytometry after culture. The CFU-Mix colony formation from CD34(+) cells was assayed. The results showed that: (1) The yield of CD34(+) cells after being selected by immune beads were (1.03 +/- 0.74)% out of bone marrow nuclear cells with purity of 86% - 93%. (2) PNS (10 - 25 mg/L) stimulated the proliferation of CD34(+) cells, and raised the colony numbers of CFU-Mix obviously in vitro. PNS 25 mg/L was the optimal concentration to promote proliferation of CD34(+) cells, the increasing rate of CFU-Mix colony was (34.7 +/- 16.0)%. (3) The differentiation of CD34(+) cells was induced by exposure to PNS (25, 50 and 100 mg/L) in liquid culture for 14 days. The percentages of CD33(+) and CD15(+) cells were increased after PNS exposure, which were significantly higher than those of control (P < 0.01), however CD71(+) and G-A(+) cells were no obviously difference after PNS treatment. In conclusion, Panax notoginosides not only promote the proliferation of CD34(+) cells, but also induce the differentiation committed to granulocytes.
