Analysis of immunophenotype of B-lineage acute lymphoblastic leukemia cells by 4-color flow cytometry.
- Author:
Hui WANG
1
;
Yan-Rong LIU
;
Shan-Shan CHEN
;
Yan CHANG
;
Ya-Zhen QIN
;
Jin-Lan LI
;
Jia-Yu FU
;
Guo-Rui RUAN
Author Information
1. Institute of Hematology, People's Hospital, Peking University, Beijing 100044, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Age Factors;
Aged;
Aged, 80 and over;
Burkitt Lymphoma;
immunology;
Child;
Child, Preschool;
Female;
Flow Cytometry;
methods;
Fusion Proteins, bcr-abl;
genetics;
Humans;
Immunophenotyping;
Leukocyte Common Antigens;
analysis;
Male;
Middle Aged;
Neprilysin;
analysis
- From:
Journal of Experimental Hematology
2003;11(2):137-141
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the characteristics of immunophenotype of B-lineage acute lymphoblastic leukemia (B-ALL) cells in Chinese cases, B-ALL cells from 181 patients were analyzed by 4-color flow cytometry with CD45/SSC gating. The results demonstrated that the antigen expression frequencies were as follows: CD19 (100%), HLA-DR (98.9%), CD38 (88.5%), CD10 (76.8%) and CD34 (76.8%). CD117 and T antigens (CD2 and CD7) were rarely expressed. Childhood group (or= 19 years) had a higher expression rate of myeloid antigens (CD13 and/or CD33). Subtype CD10(+)/CD34(+) took the highest percentage in three age groups. Percentages of CD10(-)/CD34(+) group increased by age. Subtype CD10(-)/CD34(+) had the highest myeloid antigen co-expression. Comparing with CD45-positive cases, those with CD45-negative or CD45(+/-) always had higher CD10 expression. Bcr/abl mRNA was evaluated in 43 samples from patients with B-ALL. Myeloid antigen co-expression was not different in bcr/abl(+) and bcr/abl(-) groups, and m-bcr/abl(+) was mainly observed in subtype CD10(+)/CD34(+). In conclusion, typical B-ALL cells expressed CD19(+), HLA-DR(+), and CD117(-). CD34, CD10 and CD45 expression varied in different maturation stages. The immunophenotypic subtype of B-ALL in adolescents was similar to that in the adults. Blasts with CD45(+) were mostly seen in CD10(-) cases. CD13 and/or CD33 co-expression was fewer in children patients. m-bcr/abl(+) was mainly seen in subtype CD10(+)/CD34(+).
