Application of double immunomagnetic positive sorting to ex vivo expansion of marrow CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria.
- Author:
Juan XIAO
1
;
Yong-Ji WU
;
Zhi-Nan ZHANG
;
Zhao-Jiang LU
;
Shi-Ping CHEN
;
Hong-Yan DONG
Author Information
1. Department of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Bone Marrow Cells;
cytology;
CD59 Antigens;
analysis;
Flow Cytometry;
Hematopoietic Stem Cell Transplantation;
Hemoglobinuria, Paroxysmal;
therapy;
Humans;
Immunomagnetic Separation;
Transplantation, Autologous
- From:
Journal of Experimental Hematology
2003;11(2):179-183
- CountryChina
- Language:Chinese
-
Abstract:
Since flow cytometry was not feasible for sorting a huge amount of cells for clinical use, the method of double immunomagnetic positive sorting was used for selection of CD34(+)CD59(+) cells from bone marrow mononuclear cells in patients with paroxysmal nocturnal hemoglobinuria (PNH), which laid the groundwork for clinical ABMT/APBSCT of patients with PNH. Immunomagnetic positive selection was used for two times, the microbeads were removed from the CD34(+) cells selected firstly by means of overnight culture, then the sufficient CD34(+)CD59(+) cells were used for ex vivo expansion. The results showed that the survival, proliferation and colony-forming units of the selected CD34(+)CD59(+) cells by double immunomagnetic positive sorting had no significant difference as compared with that of CD34(+)CD59(+) cells selected by flow cytometry technique. It is suggested that the double immunomagnetic positive sorting promotes the use for separation and purification hematopoietic stem/progenitor cells and other cells with double or multiple markers cells for autologous hematopoietic stem cell transplantation in PNH patients.
