Effect of angiotensin II on cord blood CD34(+) cells expansion in vitro.
- Author:
Cheng PENG
1
;
Wei-Min LI
;
Yan-Ping MA
;
Zhong-Bo HU
;
Fan-Jun CHENG
;
Ling-Bo LIU
;
Ping ZOU
Author Information
1. Institute of Hematology, The Affiliated Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China. questionpc78@sohu.com
- Publication Type:Journal Article
- MeSH:
Angiotensin II;
pharmacology;
Antigens, CD34;
blood;
Cell Differentiation;
drug effects;
Cell Division;
drug effects;
Cells, Cultured;
Colony-Forming Units Assay;
Dose-Response Relationship, Drug;
Drug Synergism;
Erythroid Precursor Cells;
cytology;
drug effects;
Erythropoietin;
pharmacology;
Fetal Blood;
cytology;
drug effects;
immunology;
Granulocyte Colony-Stimulating Factor;
pharmacology;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Hematopoietic Stem Cells;
cytology;
drug effects;
Humans;
Interleukin-3;
pharmacology;
Stem Cell Factor;
pharmacology;
Thrombopoietin;
pharmacology
- From:
Journal of Experimental Hematology
2003;11(3):227-229
- CountryChina
- Language:Chinese
-
Abstract:
Angiotensin II (Ang II) is one active substance of renin-angiotensin system. In order to explore the effect of Ang II combined with various cytokines on proliferation and differentiation of cord blood CD34(+) cells, in vitro experiments of cell cultures of Ang II with or without cytokines were taken place. The results showed that Ang II stimulated both BFU-E and CFU-GM expansion. The numbers of BFU-E and CFU-GM raised with increase of Ang II concentrations ranged from 0.01 - 0.1 micro mol/L. In semi-solid culture assay, Ang II stimulated CFU-GM production but no effect on BFU-E occurred. The multiple number of CFU-GM increased from 2.3 +/- 0.8 to 7.8 +/- 1.9 times when Ang II was added into SCF + G-CSF + GM-CSF + IL-3 combination. Similarly, the multiple number of BFU-E increased from 3.1 +/- 1.8 to 9.2 +/- 2.3 times when Ang II was combined with SCF + EPO + TPO + IL-3. In conclusion, Ang II stimulated cord blood hematopoietic stem/progenitor cell expansion in vitro the in presence of various cytokines.
