Expression of nuclear factor-kappaB in bone marrow cells from patients with acute leukemia and its relationship with P21, MMP-2 and MMP-9.
- Author:
Hai-Yan HU
1
;
Hui SUN
;
Dian-Bin ZOU
;
Ling SUN
;
Lin ZHANG
;
Ying-Lan JIA
Author Information
1. Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Adult;
Bone Marrow Cells;
metabolism;
Female;
Humans;
Immunohistochemistry;
Leukemia;
drug therapy;
metabolism;
pathology;
Male;
Matrix Metalloproteinase 2;
biosynthesis;
Matrix Metalloproteinase 9;
biosynthesis;
NF-kappa B;
biosynthesis;
Proto-Oncogene Proteins p21(ras);
biosynthesis;
Remission Induction
- From:
Journal of Experimental Hematology
2003;11(3):243-245
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression of NF-kappaB in acute leukemia and its relationship with P21, and matrix metalloproteinases (MMP), the expression of NF-kappaB, P21, MMP-2 and MMP-9 in bone marrow cells from patients with acute leukemia (AL) was detected using immunocytochemical technique. The results showed that the expression ratios of NF-kappaB, P21, MMP-2 and MMP-9 in untreated AL group were significantly higher than those in remission and normal control groups (P < 0.05), and no obvious difference was seen between remission and normal control groups. The expression of NF-kappaB was correlated with that of P21, MMP-2 and MMP-9 (r = 0.767, 0.729 and 0.803, respectively, P < 0.05). This study indicated that P21 protein, encoded by oncogene Ras, and NF-kappaB were super-expressed in leukemia cells. In conclusion, after activation by Ras, NF-kappaB combined with the kappaB sequences of MMP-2 and MMP-9 genes, then upregulated their expression. MMP might enhance the degradative function of leukemic cell, thus to make cells easier to cross through the bone marrow barrier and release into blood.
