Study on expression of cell surface L-selectin and soluble L-selectin in patients with acute leukemia.
- Author:
Jian-Hua CHANG
1
;
Zhen-Hua QI
;
Fang-Ping CHEN
;
Qin-Zhi XIE
Author Information
1. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China. changjianhua@163.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Bone Marrow Cells;
metabolism;
Child;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
L-Selectin;
blood;
genetics;
Leukemia, Myeloid, Acute;
genetics;
pathology;
Leukocytes, Mononuclear;
metabolism;
Male;
Middle Aged;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
pathology;
RNA, Messenger;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Solubility
- From:
Journal of Experimental Hematology
2003;11(3):251-255
- CountryChina
- Language:Chinese
-
Abstract:
To study the relationship between the level of the soluble L-selectin (sL-selectin) in plasma and surface L-selectin expression on leukemic cells and episode and state of illness in acute leukemia patients, the plasma level of sL-selectin was measured by a sandwich enzyme-linked immunosorbent assay, and the expressions of surface L-selectin and its gene (lyam-1) were detected by immunohistochemistry and RT-PCR. The results showed that the levels of sL-selectin were significantly higher in untreated and therapy-resistant acute leukemia patients, and expression of L-selectin mRNA and cell surface L-selectinin in untreated and NR patients were significantly lower than that in CR patients and control group (P < 0.05). The plasma levels of sL-selectin were significantly increased in patients with splenomegaly and hepatomegaly (extramedullary infiltration). The levels of sL-selectin were related to the clinical course of the acute leukemia patients. A significant correalation existed between expressions of L-selectin mRNA and surface L-selectin in acute leukemia patients (gamma = 0.782, P < 0.05). It is concluded that expression of L-selectin gene was down-regulated in level of mRNA and protein in acute leukemia patients and both changes were highly correlated. Monitoring of the plasma level of sL-selectin is possibly useful for early diagnosis of relapse and extramedullary infiltration in acute leukemia.
