Development of the human/rat chimera model with neonatal rats.
- Author:
Yi-Kun ZHANG
1
;
Dong-Mei WANG
;
Hong-Feng YUAN
;
Hai-Min LI
;
Ci-Xian BAI
;
Rui ZHANG
;
Lin CHEN
;
Suo-Qin TANG
;
Xue-Tao PEI
Author Information
1. Department of Stem Cell Biology, Institute of Transfusion Medicine, Beijing, 100850 China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Cord Blood Stem Cell Transplantation;
DNA;
genetics;
Flow Cytometry;
Humans;
Leukocyte Common Antigens;
blood;
Polymerase Chain Reaction;
Rats;
Rats, Sprague-Dawley;
Spleen;
metabolism;
Transplantation Chimera;
blood;
genetics;
immunology;
Transplantation, Heterologous;
beta 2-Microglobulin;
genetics
- From:
Journal of Experimental Hematology
2003;11(3):297-300
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to transplant neonatal rat with human cord blood Lin(-) cells to test the possibility of this xenograft model. The Lin(-) cells were purified from human cord blood (CB) using negative selection strategy based on different lineage-specific antigens. The Lin(-) cells were injected into the liver of neonatal rats using a microinjector at an average of 5 x 10(5) cells for each. Peripheral blood (PB) and spleen were collected at 2,4 and 8 weeks after injection. Flow cytometry was performed to detect human cells in the rat PB, PCR was used to detect human cells in PB as well as spleen. The results showed that a definite proportion of human cells existed in peripheral blood of chimeric rat and the human specific beta2 microglobulin gene fragments were detected in spleen genomic DNA of chimeric rat. It is concluded that human/rat chimera model can be developed with neonatal rats. Human/rat xenograft model may provide a useful and convenient method for human hematopoietic stem cell assay in vivo.
