Ex vivo expansion of CD34+ cells from human umbilical cord blood by FL gene transfected bone marrow stromal cells.
- Author:
Gang LIU
1
;
Yi ZHANG
;
Yuan-Lin LIU
;
Jin HE
;
Xiao-Xia JIANG
;
Ning MAO
Author Information
1. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Cell Separation;
Fetal Blood;
cytology;
Hematopoietic Stem Cells;
cytology;
Humans;
Interleukin-3;
pharmacology;
Membrane Proteins;
physiology;
Stem Cell Factor;
pharmacology;
Transfection
- From:
Journal of Experimental Hematology
2003;11(4):341-344
- CountryChina
- Language:Chinese
-
Abstract:
The object of this study was to explore the effect of FL gene transfected marrow stromal cells on expansion of human cord blood CD34(+) cells ex vivo. A co-culture system was established with FL gene transfected human marrow stromal cells and cord blood CD34(+) cells. The CD34(+) cells were cultured in different culture systems with different combinations of stromal cells and SCF, IL-3 and FL. The numbers of total nucleated cells, CFCs and CD34(+) cells were repeatedly counted in culture systems for 3 weeks. The results showed that the FL transgenic marrow stromal cell feeder layer significantly enhanced the expansion of total nucleated cells, CFC and CD34(+) cells, compared with stromal cell feeder layer and stromal cell-free culture systems. The transgenic stromal cells and cytokines co-culture system manifested the most potent expansion of the cord blood cells with increase of the number of total nucleated cells by (122.5 +/- 4.3)-fold, CFCs by (39.6 +/- 2.7)-fold and CD34(+) cells by (11.8 +/- 0.52)-fold. It is concluded that human umbilical cord blood CD34(+) cells can be extensively expanded ex vivo by using FL gene transfected stromal cells combined with cytokines.
