The effects of rhG-CSF and rhSCF on peripheral blood leukocytes and CFU-GM in rhesus monkeys.
- Author:
Jing HAO
1
;
Guo-Lin XIONG
;
Xiao-Lan LIU
;
Zhen-Hu ZHAO
;
De-Huang GUO
;
Bei-Lei ZHAO
;
Xin-Ru WANG
;
Song-Sen CHEN
;
Peng-Yi LUO
;
Qing-Liang LUO
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Drug Therapy, Combination;
Female;
Granulocyte Colony-Stimulating Factor;
administration & dosage;
pharmacology;
Hematopoietic Stem Cells;
drug effects;
Leukocyte Count;
Leukocytes;
drug effects;
Macaca mulatta;
Male;
Recombinant Proteins;
pharmacology;
Stem Cell Factor;
administration & dosage;
pharmacology
- From:
Journal of Experimental Hematology
2003;11(4):381-384
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the effects of rhG-CSF and rhSCF on mobilization of the peripheral blood stem cells, 15 monkeys were divided into control, rhG-CSF 10 micro g/(kg x day) and rhG-CSF 10 micro g/(kg x day) + rhSCF 50 micro g/(kg x day) treated groups. Monkeys were administered with vehicle, rhG-CSF and rhG-CSF + rhSCF subcutaneously once daily for 14 days, respectively. The results showed that the highest counts of leukocyte of rhG-CSF treated group were 411% of baseline value on day 7 after administration, compared with that of rhG-CSF + rhSCF treated group which were 538% on day 9. The highest counts of leukocytes lasted for 3 days in combined treated group. CFU-GM from peripheral blood in the two groups were 8.37 and 11.75 times higher at 5 and 9 days respectively after the mobilization. It is concluded that rhG-CSF significantly increases the number of peripheral blood leukocytes and CFU-GM, and a better effect can be obtained by rhSCF + rhG-CSF combined administration.
