Effects of Chinese drugs for activating blood and Chinese drugs for nourishing qi and activating blood on the metastasis of Lewis lung carcinoma in different stages.
- Author:
Sheng LIU
1
;
Li-li HE
;
Li-na ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Lewis Lung; drug therapy; immunology; pathology; Drugs, Chinese Herbal; pharmacology; therapeutic use; Forkhead Transcription Factors; metabolism; Interferon-gamma; immunology; Interleukin-17; immunology; Interleukin-23; immunology; Male; Mice; Mice, Inbred C57BL; Neoplasm Metastasis; Nuclear Receptor Subfamily 1, Group F, Member 3; metabolism; T-Lymphocytes, Helper-Inducer; immunology; T-Lymphocytes, Regulatory; immunology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(12):1638-1641
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo reveal the different molecular mechanisms between Chinese drugs for activating blood (CDAB) and Chinese drugs for nourishing qi and activating blood (CDNQAB) in the metastasis process of Lewis lung carcinoma, thus providing experimental reliance for Chinese drugs to reverse immune escape.
METHODSThe inhibition rate of lung metastasis was observed in each group. The dynamic percentage and ratio changes of Th17 and Treg cells in spleen CD4+ T lymphocytes were detected using flow cytometry. The dynamic levels of IL-17, IL-23, and gamma interferon (IFN-gamma) in the culture supernatant of CD4+ T lymphocytes were detected by ELISA. The dynamic mRNA expressions of Foxp3 and RORgammat in CD4+ T lymphocytes were detected by RT-PCR.
RESULTSCDNQAB (sapanwood +astragalus) showed better lung metastasis inhibiting rate than CDAB (sapanwood alone) (P<0.05), similar to the effects of cyclophosphamide (P>0.05). Except the CDNQAB group, spleen Th17 and Treg cells showed a rising tendency in mice of each tumor-bearing group. The effectors of Th17 and Treg cells (IL-17, IL-23, and IFN-gamma) and key transcription molecules of Th17 and Treg cells (RORgammat and Foxp3) showed dynamic changes corresponding to Th17 and Treg cells.
CONCLUSIONSThe immune inflammatory reactions of CDNQAB (sapanwood +astragalus) were superior to those of CDAB (sapanwood alone) and of cyclophosphamide during the process of inhibiting tumor immunotolerance and of the formation of tumor. All drugs showed certain inhibition on the mechanisms for neoplasm metastasis. But CD-NQAB was superior to CDAB and chemotherapeutics.
