Design of a microfluidic immunoassay system based on superparamagnetic microbeads.
- Author:
Hui HUANG
1
;
Xiao-Lin ZHENG
;
Jing PAN
;
Zhi-Qiang ZHAO
;
Xiao-Yun PU
Author Information
1. Key Lab of Biomechanics and Tissue Engineering, State Education Ministry, Bioenginering College, Chongqing University, Chongqing.
- Publication Type:Journal Article
- MeSH:
Equipment Design;
Humans;
Immunoassay;
instrumentation;
methods;
Luminescent Measurements;
instrumentation;
methods;
Microfluidic Analytical Techniques;
instrumentation;
alpha-Fetoproteins;
analysis
- From:
Chinese Journal of Medical Instrumentation
2006;30(5):369-371
- CountryChina
- Language:Chinese
-
Abstract:
We use direct-write laser micromachining technology to fabricate the microfluidic chip, and to establish a microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads, for detecting alpha- fetoprotein (AFP). The AFP analysis can be completed in 20 minutes with 5 microl sample and 5 microl reagent, and there is a good linear correlation in the range of 1-800 ng/ml.
