Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells.
- Author:
Jianli SHAO
1
;
Zhizhong LI
;
Genlong JIAO
;
Guodong SUN
;
Zhigang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Bone Neoplasms; pathology; Cell Line, Tumor; drug effects; Cell Movement; drug effects; Cell Proliferation; drug effects; Heptanoic Acids; pharmacology; Humans; Lanosterol; analogs & derivatives; pharmacology; NF-kappa B p50 Subunit; metabolism; Osteosarcoma; pathology; Phosphorylation; STAT3 Transcription Factor; metabolism; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Journal of Southern Medical University 2015;35(5):619-624
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro.
METHODSMG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting.
RESULTSGA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells.
CONCLUSIONGA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.
