Lentivirus-mediated angiotensin-converting enzyme 2 overexpression inhibits angiotensin II-induced albumin down-regulation and enhancement of migration in rat hepatocytes.
- Author:
Lili ZHANG
1
;
Wenyong ZHANG
;
Yang LI
;
Xu LI
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; metabolism; Angiotensin II; pharmacology; Animals; Cell Movement; Cells, Cultured; Down-Regulation; Hepatocytes; cytology; Lentivirus; Peptidyl-Dipeptidase A; genetics; metabolism; Rats; Transfection; Up-Regulation; Vimentin; metabolism
- From: Journal of Southern Medical University 2015;35(5):652-658
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of angiotensin-converting enzyme 2 (ACE2) on angiotensin II (Ang II)-induced down-regulation of albumin expression and enhancement of cell migration in rat hepatocytes.
METHODSCultured rat hepatocyte were treated with Ang II (10-7 mol/L) for different time lengths, and the protein expressions of vimentin and albumin and cell migration were detected. The cells transfected with lentiGFP or lentiACE2 were treated with A779 for 1 h and then with Ang II, and Western blotting and immunofluorescent cytochemistry were used to detect the protein levels; the cell migration was evaluated by Transwell assay.
RESULTAng II induced significantly increased vimentin expression and reduced albumin expression in rat hepatocytes in a time-dependent manner. Overexpression of ACE2 obviously inhibited the up-regulation of vimentin expression, reduction of albumin expression, and enhancement of cell migration induced by Ang II.
CONCLUSIONACE2 overexpression can inhibit Ang II-induced up-regulation of vimentin, reduction of albumin expression, and enhancement of cell migration in rat hepatocytes.
