Effects of chloroquine in reversing multidrug resistance in HNE1/DDP cell line.
- Author:
Haoxuan ZHANG
1
;
Xiaojin SUN
;
Yiming SUN
;
Surong ZHAO
;
Chenchen JIANG
;
Hao LIU
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; metabolism; Antineoplastic Agents; pharmacology; Apoptosis; Cell Line, Tumor; Chloroquine; pharmacology; Down-Regulation; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Humans
- From: Journal of Southern Medical University 2015;35(5):687-691
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of chloroquine in reversing multidrug resistance (MDR) of HNE1/DDP cell line and explore the mechanism.
METHODSMTT assay was used to detect the cell viability of HNE1 and HNE1/DDP after exposure to different concentrations of DDP (2, 4, 8, 16 and 32 µmol/L) and different concentrations of chloroquine (5, 10, 20, 40, and 80 µmol/L). q-PCR was used to assess the expression of MDR1 mRNA and Western blotting was employed to detect P-glycoprotein (P-gp) expression in HNE1 and HNE1/DDP cells exposed to 5 and 10 µmol/L chloroquine. The cell apoptosis rate of HNE1 and HNE1/DDP cells exposed to 10 and 20 µmol
RESULTSChloroquine exposure caused dose-dependent suppression of the proliferation in both HNE1 and HNE1
CONCLUSIONChloroquine can reverse multidrug resistance in HNE1
