Detection of serum antibodies against Japanese encephalitis virus in bats in Hainan and Guangdong Provinces of China.
- Author:
Lina JIANG
1
;
Shaowei CHEN
;
Xueyan ZHENG
;
Shujuan MA
;
Junhua ZHOU
;
Qionghua ZHANG
;
Xing LI
;
Yiquan XIONG
;
Xueshan ZHONG
;
Zhiyun WANG
;
Qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; China; Chiroptera; immunology; virology; Encephalitis Virus, Japanese; Enzyme-Linked Immunosorbent Assay; Neutralization Tests
- From: Journal of Southern Medical University 2015;35(5):720-723
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the prevalence of serum antibodies against Japanese encephalitis virus (JEV) in bats.
METHODSBlood samples from the heart were obtained from bats captured in Guangdong and Hainan Provinces in 2013. The anti-JEV antibodies in bat sera were tested using indirect ELISA and virus neutralization test.
RESULTSA total of 201 bat serum samples were tested, in which the total positivity rate of anti-JEV antibodies was 46.27% (93/201). The positive rate of anti-JEV antibodies in bats from Hainan and Guangdong Provinces was 88.89% (48/54) and 30.61% (45/147), respectively. All the samples from Rousettus leschenaultia, Miniopterus schreibersii, Pipistrellus abramus, and Rhinolophus macrotis were positive for anti-JEV antibodies, and up to 95.56% (43/45) of the samples from Miniopterus schreibersii (from Hainan Province) yielded positive results. Of the 28 samples with positive results by indirect ELISA, 15 showed positive results in virus neutralization test (53.57%) with neutralization antibody titers ranging from 1:10 to 1:28.22.
CONCLUSIONBats from different regions and of different species can be naturally infected with JEV and have a high prevalence of anti-JEV antibodies in their sera. The role of bats in the natural cycle of JEV awaits further study.
