Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells.
- Author:
Chao FAN
1
;
Mingyu FU
;
Zhongju XIAO
;
Jie TANG
Author Information
- Publication Type:Journal Article
- MeSH: Anion Transport Proteins; metabolism; HEK293 Cells; Humans; Patch-Clamp Techniques; Transfection
- From: Journal of Southern Medical University 2015;35(6):801-811
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo express solute carrier 26A proteins in HEK-293 cells and explore their functions.
METHODSSLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing SLC26A proteins was measured by whole-cell patch recording.
RESULTSAll the SLC26A transporters were expressed on the membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which represented their binding capability with anions.
CONCLUSIONThe SLC26A transporters expressed on HEK cells show similar functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A transporters.
