Expression of BATF, a member of the activator protein-1 family, in renal tissues of MRL/lpr mice.
- Author:
Yusheng PENG
1
;
Qian LI
;
Menglei WANG
;
Can CAO
;
Kuan LAI
;
Kang ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basic-Leucine Zipper Transcription Factors; metabolism; Blotting, Western; Female; Interleukin-17; metabolism; Kidney; metabolism; Kidney Glomerulus; pathology; Lupus Nephritis; metabolism; Mice; Mice, Inbred C57BL; Mice, Inbred MRL lpr; Nuclear Receptor Subfamily 1, Group F, Member 3; metabolism; RNA, Messenger; Real-Time Polymerase Chain Reaction
- From: Journal of Southern Medical University 2015;35(6):816-819
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of BATF, a member of the activator protein-1 family, in the renal tissues of mice with lupus nephritis.
METHODSThe renal tissues from 24-week-old female MRL/lpr mice and age- and sex-matched C57BL/6 mice were examined for BATF protein expressions using Western blotting and for expressions of BATF, RORγt and IL-17A mRNA using quantitative real-time PCR. The results of laboratory examinations were analyzed in relation with the histopathology of the mice.
RESULTSThe urinary protein and ds-DNA levels were significantly higher in MRL/lpr mice than in the control mice (P<0.05). Compared to normal control mice, MRL/lpr mice showed obvious glomerular fibrosis and inflammatory cell infiltrating with significantly increased BATF protein and mRNA expressions (P<0.05) and RORγt and IL-17 mRNA expressions in the renal tissues (P<0.05). In MRL/lpr mice, the expression of BATF mRNA was positively correlated with the RORγt and IL-17A mRNA expressions in the renal tissues.
CONCLUSIONThe renal expressions of BATF protein and mRNA is increased in MRL/lpr mice. BATF may participate in the immunopathogenesis of lupus nephritis by enhancing Thl7 cell response.
