Effect of RNA interference of CD59 gene on proliferation of non-small cell lung cancer cell line GLC-P in vitro.
- Author:
Weidong WU
1
;
Dan LIU
;
Wenjin HOU
;
Yongsheng YI
;
Yuejun WANG
;
Weijia LIN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; CD59 Antigens; genetics; Carcinoma, Non-Small-Cell Lung; pathology; Cell Line, Tumor; Cell Proliferation; Enzyme-Linked Immunosorbent Assay; Genetic Therapy; Humans; RNA Interference; RNA, Messenger; RNA, Small Interfering; Transfection
- From: Journal of Southern Medical University 2015;35(6):903-906
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of CD59 gene inhibition mediated by RNA interference on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) GLC-P cells in vitro.
METHODSRecombinant plasmids for RNA interference of CD59 gene were constructed and transfected into GLC-P cells via lipofectamine 2000. The stably transfected cells were examined with real-time RT-PCR, MTT assay and enzyme-linked immunosorbent assay to investigate the changes in cell proliferation and apoptosis.
RESULTSCompared with the control cells, the cells transfected with CD59-siRNA showed significantly decreased expression levels of CD59 mRNA (P<0.05) and significantly inhibited cell proliferation.
CONCLUSIONCD59 gene is highly expressed in NSCLC and RNA interference-mediated CD59 silencing can strongly inhibit the proliferation and induce apoptosis in GLC-P cells, which shed light on a potentially new target for targeted gene therapy of NSCLC.
