Tissue engineered bone reconstruction with modified PLGA/Type-I collagen compound scaffold.
- Author:
Hui-yong ZHU
1
;
Hui-ming WANG
;
Qiu-liang WU
;
Ying-qian HU
;
Jian-hua LIU
;
Kang-jie ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Absorbable Implants; Animals; Biocompatible Materials; Cells, Cultured; Collagen Type I; therapeutic use; Female; Femur; cytology; Lactic Acid; therapeutic use; Male; Osteoblasts; cytology; Polyglycolic Acid; therapeutic use; Polymers; therapeutic use; Prostheses and Implants; Rabbits; Stents; Stromal Cells; cytology; transplantation; Tissue Engineering
- From: Journal of Zhejiang University. Medical sciences 2005;34(3):233-236
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo fabricate bone grafts by bone marrow stromal cell combined with modified PLGA/Type-I collagen compound scaffold using tissue engineering method.
METHODSThe modified PLGA/Type-I collagen compound scaffold was fabricated. The rabbit primary cultured osteoblasts were identified and seeded onto the modified compound scaffold for one week in vitro. The adhesion and growth of cells were observed with scanning electron microscope. The complex of cells and scaffold was implanted into the subcutaneous region of rabbits and new bone formation was evaluated.
RESULTSThe rabbit bone marrow stromal cells were induced and differentiated into osteoblasts. The adhesion and growth of osteoblasts in cluster were observed on the surface of scaffolds. New bone formation was observed at one month postoperatively and active osteoblasts were found on the surface of the newly formed bone in vivo.
CONCLUSIONThe complex of PLGA and type-I collagen is an appropriate biodegradable scaffold and can be applied in bone tissue engineering.
