Mycophenolic acid induced apoptosis in T lymphocytic cells Molt-4 and its mechanism.
- Author:
Xiao-li ZHU
1
;
Li LI
;
Ji-min SHI
;
Wei-yan ZHENG
;
Jian YU
;
He HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antineoplastic; pharmacology; Apoptosis; drug effects; Caspase 3; Caspases; metabolism; Dose-Response Relationship, Drug; Humans; Leukemia, T-Cell; pathology; Mycophenolic Acid; pharmacology; Time Factors; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2005;34(4):339-343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether mycophenolic acid (MPA) exerts an apoptotic effect on human leukemic T cells Molt-4 and to elucidate its possible mechanism.
METHODSCell morphology, DNA fragmentation, cell cycle,percentage of annexin V positive cells and enzymatic activity of caspase-3 were measured by microscopic, electrophoretic and flow cytometric techniques respectively. Human leukemic B cell line Raji was used as control.
RESULTMPA could induce apoptosis of Molt-4 cells with dose-and time-dependent manners; cells were blocked in the S phase. The activity of caspase-3 was enhanced in a dose-dependent manner in Molt-4 cells treated with MPA for 24 h. MPA could not induce apoptosis in Raji cells.
CONCLUSIONMPA can induce apoptosis in Molt-4 cells which may be involved in the activation of caspase-3.