Photomacrography of brain surface for evaluating blood-brain barrier disruption within 24 h after focal cerebral ischemia in mice.
- Author:
Li-ping CHEN
1
;
Hui-min XU
;
Wei ZHAO
;
Shi-hong ZHANG
;
Zhao-yang ZHU
;
Qi ZHANG
;
Guo-liang YU
;
Sheng-li CHU
;
Er-qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood-Brain Barrier; physiopathology; Brain; physiopathology; Evans Blue; Infarction, Middle Cerebral Artery; physiopathology; Male; Mice; Mice, Inbred ICR; Photography; Time Factors
- From: Journal of Zhejiang University. Medical sciences 2005;34(6):523-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new photomacrographic analysis of morphological changes on brain surface to evaluate blood-brain barrier (BBB) disruption.
METHODSPermanent focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in mice. Brains were removed 10 min, 0.5, 1, 3, 6, 12 and 24 h after MCAO. The whole brains and brain slices were photographed by a digital camera. BBB disruption was evaluated by hemorrhage and traced Evans blue (EB) on the brain surface. Fluoremetric quantitation of EB and water content in the brains were also performed at various time points.
RESULTPhotomacrographic morphological analysis showed that hemorrhage and traced EB on the surface of the brains significantly increased from 3 h after focal cerebral ischemia,which were correlated to the results in the brain slices. EB content in the ischemic hemispheres was significantly increased from 0.5 h after MCAO, and water content was increased from 1 h after MCAO.
CONCLUSIONPhotomacrographic measurement is a simple and useful method for evaluating BBB disruption semi-quantitatively, and can detect BBB disruption earlier after focal cerebral ischemia in mice.
