Telomerase activity and telomere length in CD4+,CD8+ and CD19+ lymphocytes from patients with systemic lupus erythematosus.
- Author:
Jin LIN
1
;
Jue XIE
;
Wen-bin QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Antigens, CD19; immunology; CD4-Positive T-Lymphocytes; enzymology; CD8-Positive T-Lymphocytes; enzymology; Female; Humans; Lupus Erythematosus, Systemic; enzymology; immunology; Lymphocytes; enzymology; Male; Middle Aged; Telomerase; metabolism; Telomere; genetics
- From: Journal of Zhejiang University. Medical sciences 2005;34(6):534-537
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the telomerase activity and the telomere length in CD4(+), CD8(+) and CD19(+) lymphocytes from patients with systemic lupus erythematosus (SLE).
METHODSTelomerase activity of CD4(+), CD8(+) and CD19(+) cells from patients with SLE and normal controls was examined by telomeric repeats amplification protocol. Telomere length in those cells was measured by Southern blot method.
RESULTCD4(+), CD8(+) and CD19(+) cells in patients with SLE showed high telomerase activity, but only telomerase activity of CD19(+) cells was significantly higher than that in controls. The length of telomere in CD4(+) and CD8(+) cells was significantly shorter than that in controls, but not in CD19(+) cells.
CONCLUSIONHigher telomerase activity in CD19(+) cells and shortened telomere length in CD4(+) and CD8(+) cells of patients with SLE may be associated with pathogenesis of SLE.
