Protection of Hsian-tsao (Mesona procumbens Hemsl)on H2O2-induced DNA damage of primarily cultured mice spleen lymphocytes.
- Author:
Min YANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA Damage; drug effects; Drugs, Chinese Herbal; pharmacology; Female; Hydrogen Peroxide; Lamiaceae; Lymphocytes; cytology; metabolism; Male; Mice; Protective Agents; pharmacology; Spleen; cytology
- From: Journal of Zhejiang University. Medical sciences 2006;35(1):34-38
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVETo investigate the protection of Hsian-tsao(Mesona procumbens Hemsl)on H(2)O(2)-induced DNA damage of primarily cultured spleen lymphocytes.
METHODSSpleen lymphocytes were primarily cultured for 24 h and then divided into 6 groups randomly: 4 groups were treated with different concentrations of Hsian-tsao extracts for 60 min, then with 50 micromol/L H(2)O(2); 1 group was treated with 50 micromol/L H(2)O(2) only and the last group with PBS. Twenty min after H(2)O(2)or PBS treatment SCGE and laser confocal microscopy were performed, then the comet length and percentage of cells with migrated DNA were measured.
RESULTSH(2)O(2) caused severe DNA damage in primarily cultured spleen lymphocytes and Hsian-tsao extracts reduced the DNA damage at a concentration of 10, 50 and 100 microg/ml. The quantity of comet cells was reduced from 100% to 88%, 60%, 36% (P<0.05, 0.01, 0.01) and the comet length was reduced from (49.56+/-6.94) microm to (41.14+/-5.64) microm, (38.89+/-9.81) microm, (28.62+/-4.66) microm (P<0.05, 0.01, 0.01), respectively.
CONCLUSIONHsian-tsao has dramatic ability of antioxidant. It can protect cells from the damage of ROS-induced DNA injury in the certain range.
