Activity of the TNF-related apoptosis-inducing ligand gene expressed from the hTERT promoter on colon cancer cell line HT-29.
- Author:
Bin CHEN
1
;
Chao HE
;
Wei-feng LAO
;
Xue-feng HUANG
;
Bing-liang FANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Apoptosis; physiology; Colonic Neoplasms; enzymology; pathology; Genetic Vectors; Green Fluorescent Proteins; biosynthesis; genetics; HT29 Cells; Humans; Promoter Regions, Genetic; genetics; TNF-Related Apoptosis-Inducing Ligand; biosynthesis; genetics; Telomerase; genetics; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2006;35(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the expression and activity of TNF-related apoptosis-inducing ligand (TRAIL) gene expressed from the hTERT promoter on colon cancer cell line HT-29.
METHODSGFP/TRAIL gene expressed from the hTERT promoter was transfected into HT-29 with adenoviral vectors system, expression and apoptosis inducing ability of GFP/TRAIL protein were determined with fluorescence-activated cell sorting (FACS) method.
RESULTSThe expression of GFP gene was 31.4 % and 67.0 % with either hTERT promoter or CMV promoter in DLD1 cells; GFP/TRAIL gene was able to inhibit cell growth (74.2%) and induce apoptosis (25.8%) of HT-29 cells. There was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/CMV-GFP, P<0.05).
CONCLUSIONThe GFP/TRAIL gene with hTERT promoter transfected by adenoviral vector was successfully expressed in HT-29 cell, which can both inhibit cell growth and induce apoptosis of colon cancer cell line HT-29.
