Genotypes of aminoglycoside-modifying enzyme and clinical study of high-level gentamycin resistant enterococcus.
- Author:
Ting-ting QU
1
;
Ying ZHANG
;
Yun-song YU
;
Ya-gang CHEN
;
Ze-qing WEI
;
Lan-juan LI
Author Information
- Publication Type:Journal Article
- MeSH: Drug Resistance, Bacterial; genetics; Electrophoresis, Gel, Pulsed-Field; Enterococcus; drug effects; genetics; Enterococcus faecalis; drug effects; genetics; Enterococcus faecium; drug effects; genetics; Gentamicins; pharmacology; Humans; Kanamycin Kinase; genetics; Microbial Sensitivity Tests
- From: Journal of Zhejiang University. Medical sciences 2006;35(1):76-82
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the antibiotics resistance, aminoglycoside-modifying enzymes and homology of high-level gentamycin resistant enterococcus in clinical specimens.
METHODSThe high-level gentamicin resistant (HLGR) isolates were screened by the agar method and the resistance of 14 antimicrobial agents was determined by K-B method. The aminoglycoside-modifying enzyme genes were detected by polymerase chain reaction (PCR). Pulsed-field gel electrophoresis (PFGE) was used to analyze the homology of HLGR isolates.
RESULTSThe ratio of HLGR was 64.2% (68/106). Among the HLGR,there were no isolates resistant to linezolid, vancomycin and tecoplanin, and Enterococcus faecium was more resistant to beta-lactam antibiotics and quinolone than Enterococcus faecalis. The positive rate of aac(6')-Ie-aph(2')-Ia was 92.6% and 3 isolates had the resistance gene mostly similar to aph(2')-Id. And among 51 HLGR isolates from the hospitalized patients, PFGE grouped 17 E. faecalis isolates into 4 clusters (A-D), and 33 E. faecium isolates into 8 clusters (A-H) with A cluster as predominant.
CONCLUSIONHLGR has become the important antibiotic resistance bacteria which results in nosocomial infection; and aac(6')-Ie-aph(2')-Ia is the main aminoglycoside-modifying enzyme gene which causes HLGR.
