Evaluation of aminoglycoside resistance phenotypes and genotyping of acetyltransferase in Escherichia coli.
- Author:
Hai-shen KONG
1
;
Xue-fen LI
;
Ju-fang WANG
;
Min-jin WU
;
Xiao CHEN
;
Qing YANG
Author Information
- Publication Type:Journal Article
- MeSH: Acyltransferases; genetics; Amikacin; pharmacology; Aminoglycosides; pharmacology; Drug Resistance, Bacterial; genetics; Escherichia coli; enzymology; genetics; Genotype; Gentamicins; pharmacology; Phenotype; Tobramycin; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2006;35(1):83-86
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the prevalence of aminoglycoside resistance and genotyping of acetyltransferase in Escherichia coli.
METHODSResistance phenotypes to 12 antibiotics of 44 Escherichia coli isolates were analyzed using agar dilution method and 3 aminoglycoside resistance genes aac(3)-I, II and aac(6')-I were determined by PCR method.
RESULTSIn 44 clinical isolates, the occurrence of ESBLs was 45.45%, resistance rates were discrepant for amikacin (18.18%), gentamicin (56.82%) and tobramycin (61.36%), the prevalence of phenotype TG (tobramycin and gentamicin) indicative of aac(3)-II production and TGA (tobramycin, gentamicin and amikacin) indicative of aac(6')-I production were 36.36% and 18.18%, respectively. The most common aminoglycoside resistance genotype of acetyltransferase was aac(3)-II (52.27%) and aac(6')-I was lower (29.55%), but no aac(3)-I was detected.
CONCLUSIONAt least 2 acetyltransferase genes exist in this area i.e. aac(3)-II and aac(6')-I.
