Preliminary evidence that a hepatitis E virus (HEV) ORF2 recombinant protein protects cynomolgus macaques against challenge with wild-type HEV.
- Author:
Shenli BI
1
;
Jian LU
;
Lin JIANG
;
Guoyong HUANG
;
Haidong PAN
;
Yongzhen JIANG
;
Mingcheng ZHANG
;
Xinliang SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Hepatitis Antibodies; blood; Hepatitis E; immunology; prevention & control; Hepatitis E virus; immunology; Macaca mulatta; Male; Recombinant Proteins; immunology; Viral Proteins; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(1):31-32
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo observe the protective effect of hepatitis E virus (HEV) ORF2 recombinant protein expressed in prokaryote cell cynomolgus macaques (cynos) against challenging with wild-type HEV.
METHODSCynos were immunized with HEV ORF2 recombinant protein and then challenged with wild-type HEV, the unimmunized cynos were used as control. Blood samples were collected and tested to see if there were dynamic changes of ALT and antibody to HEV before and after challenge with wild-type HEV.
RESULTSAll the five unimmunized cynos re-presented hepatitis 3 weeks after challenging with wild-type HEV. However, all the five immunized cynos showed no hepatitis and pathological changes.
CONCLUSIONSCynos can be efficiently protected by immunization with HEV ORF2 recombinant protein against wild-type HEV. This protein can be a promising candidate for HEV vaccine.
