Modification of HPV type 16 E6 and E7 genes, and analysis of stability and immunogenicity of the modified proteins.
- Author:
Huijun ZHI
1
;
Liqun HAN
;
Jiao REN
;
Houwen TIAN
;
Weifing LUO
;
Yu LIANG
;
Li RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; biosynthesis; Female; Mice; Mice, Inbred C57BL; Mutagenesis, Insertional; Oncogene Proteins, Viral; genetics; immunology; Papillomaviridae; genetics; Papillomavirus E7 Proteins; Repressor Proteins; Vaccinia virus; immunology; Zinc Fingers
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo select the mutants of HPV type 16 E6 and E7 genes suitable for construction of vaccine for treatment of cervical cancer.
METHODSE6 and E7 genes were modified by site-directed mutagenesis. Several recombinant vaccina viruses were constructed by inserting the E6 or E7 mutants into the genome of vaccina virus Tiantan strain and employed to study their antigenicity.
RESULTSWestern blot assay showed that the E6 ?mutant? with substitution of Gly for Leu at amino acid site 50 and E7 mutant with substitution of Gly for Cys-24 and Glu-26 had no effect on their stability and antigenicity, but change of the Cys at position 91 of E7 dramatically reduced its stability and antigencity. Conclusion The results confirmed that the Zinc-finger structure at the E7 C-terminal? plays an important role in the integrity and stability of E7 protein.
