High glucose up-regulates GRK2 gene expression in H9C2 cardiomyoblasts in vitro.
- Author:
Hong-mei CHEN
1
;
Qiu-xiong LIN
;
Hong-hong TAN
;
Hua-zhang YANG
;
Xi-yong YU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Diabetes Complications; metabolism; G-Protein-Coupled Receptor Kinase 2; genetics; metabolism; Gene Expression Regulation; Glucose; pharmacology; Humans; Myocytes, Cardiac; cytology; metabolism; RNA, Messenger; genetics; metabolism; Up-Regulation
- From: Journal of Southern Medical University 2010;30(3):472-474
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of high glucose on GRK2 gene expression in H9C2 cardiomyoblasts in vitro.
METHODSH9C2 cardiomyoblasts were cultured for 72 h in the presence of 0, 5.5, 12.5, 25 or 33 mmol/L glucose (with the osmotic pressure adjusted with monnitol). Semi-quantitative detection of GRK2 gene expression in H9C2 cardiomyoblasts was carried out using RT-PCR and phosph-Akt (Ser473) protein level was measured by Western blotting.
RESULTSGlucose in the culture medium (5.5 to 33 mmol/L) concentration-dependently increased the mRNA expression of GRK2 concentration and decreased phosphorylation Akt (ser473) level in in H9C2 cardiomyoblasts.
CONCLUSIONIncreased GRK2 gene expression may play an important role in cardiac dysfunction in diabetes.
