Neuronal differentiation of PC12 cells induced by human periodontal ligament fibroblast-conditioned medium.
- Author:
Juan XU
1
;
Hong-chen LIU
;
Lu-lu XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Cells, Cultured; Culture Media, Conditioned; pharmacology; Fibroblasts; cytology; Humans; Neurons; cytology; PC12 Cells; Periodontal Ligament; cytology; Periodontium; cytology; Pheochromocytoma; pathology; Rats
- From: Journal of Southern Medical University 2010;30(3):549-551
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine whether human periodontal ligament fibroblasts (hPDLF) express neural growth factor (NGF).
METHODSPC12 cells were cultured in hPDLF-conditioned medium to evaluate the secretion of NGF by hPDLF.
RESULTShPDLF-conditioned medium resulted in neuronal differentiation and neurite outgrowth from PC12 cells. Only a small percentage of the cells grown in anti-NGF antiserum and DMEM expressed neuritis.
CONCLUSIONhPDLF are capable of synthesizing NGF and secreting it into the medium as the signaling molecules for innervation during the healing of the periodontal tissue.
