Effect of recombinant human erythropoietin on bcl-2 protein expression in the retina in a rabbit model of acute high intraocular pressure.
- Author:
Jian-ming WANG
1
;
Yan-ping SONG
;
Nai-xue SUN
;
Na HUI
;
Shi-ping ZHAO
;
Kai HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Erythropoietin; pharmacology; therapeutic use; Female; Humans; Male; Ocular Hypertension; drug therapy; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; Rabbits; Random Allocation; Recombinant Proteins; Retina; metabolism
- From: Journal of Southern Medical University 2010;30(3):552-554
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of bcl-2 protein in the retina of rabbits with acute high intraocular pressure and explore the mechanism underlying the protective effect of rhEPO on the retina against ischemia-reperfusion injury.
METHODSrhEPO was injected subcutaneously in the ear of a rabbit model of acute high intraocular pressure induced by physiological saline perfusion into the anterior chamber. Bcl-2 protein expression in the retina of the rabbits was observed by immunohistochemical staining on days 1, 3, 7, and 14 after retinal ischemia-reperfusion and compared with that in normal rabbits and untreated rabbit models.
RESULTSbcl-2-positive cells were observed in the retina of normal rabbits with a mean positive cell number of 10.5-/+1.2 in each high-power visual field. Compared with that in the normal control group, the number of the positive cells decreased significantly in both the model group and EPO group (P<0.05, P<0.01), but the latter group showed a significantly greater number than the former (P<0.05 at day 7 and P<0.01 at day 14).
CONCLUSIONSystemic administration of rhEPO can up-regulate the expression of bcl-2 protein in the retina of rabbits with acute high intraocular pressure, which is probably one of the mechanisms for the protective effect of rhEPO on the retina against ischemia-reperfusion injury.