Effect of endogenous brain derived neurotrophic factor on GAP-43 expression in the anterior horn of the spinal cord in rats with sciatic nerve injury.
- Author:
Zhi-fei WANG
1
;
Da-guang LIAO
;
Chang-qi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anterior Horn Cells; metabolism; Brain-Derived Neurotrophic Factor; metabolism; physiology; Down-Regulation; GAP-43 Protein; genetics; metabolism; Male; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Sciatic Nerve; injuries; Spinal Cord; metabolism
- From: Journal of Southern Medical University 2010;30(3):569-571
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of endogenous brain derived neurotrophic factor (BDNF) on GAP-43 expression in the anterior horn of the spinal cord in rats following sciatic nerve injury.
METHODSBDNF antibody was injected intraperitoneally in rats with crushing injury of the sciatic nerve, and the control rats received normal saline only after sciatic nerve injury. At 7 and 14 days after the injection, the expression of GAP-43 in the anterior horn of the corresponding segments of the spinal cord was detected by Western blot and RT-PCR.
RESULTSThe expressions of GAP-43 protein and mRNA in the anterior horn of the spinal cord were significantly down-regulated in rats with BDNF antibody injection as compared with those in the control group (P<0.01).
CONCLUSIONEndogenous BDNF may regulate the expression of GAP-43 in the spinal cord anterior horn after sciatic nerve injury in rats.
