Expression of microRNA-21 in ovarian epithelial carcinoma and its clinical significance.
- Author:
Yan-hui LOU
1
;
Xing-sheng YANG
;
Fu-ling WANG
;
Jin-hua QIAN
;
Yu HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cystadenocarcinoma, Serous; genetics; metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; Lymphatic Metastasis; MicroRNAs; genetics; metabolism; Middle Aged; Ovarian Neoplasms; genetics; metabolism; Prognosis; Young Adult
- From: Journal of Southern Medical University 2010;30(3):608-613
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of microRNA-21(miR-21) in ovarian epithelial carcinoma and its association with the clinicopathological features.
METHODSThe expression of miR-21 was detected by Stem-loop real-time RT-PCR in 48 cases of ovarian epithelial carcinomas, 24 cases of benign ovarian epithelial tumors and 15 cases of normal ovarian tissues.
RESULTSThe relative expression level of miR-21(2-(DeltaDelta)CT) was 4.849-/+1.813 in the ovarian epithelial carcinomas, significantly higher than that in the benign ovarian tumors and normal ovarian tissues (P<0.01), but comparable between the latter two groups. The expression of miR-21 was not correlated to the histological type, but increased significantly with the progression of the clinical stages and histological grading (P<0.01), showing a close correlation to lymphatic metastasis.
CONCLUSIONMiR-21 might play a role as an oncogene in the tumorigenesis and development of ovarian epithelial carcinoma, and is possibly correlated to the progression and prognosis of ovarian epithelial carcinoma.
