Tumor-targeted human telomerase reverse transcriptase promoter/tk gene therapy against human nasopharyngeal carcinoma cells in vitro.
- Author:
Fang XU
1
;
Zhong WEN
;
Yuan-zheng QIU
;
Jian-yun XIAO
;
Su-ping ZHAO
;
Meng-he GUO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Gene Targeting; Genetic Therapy; methods; Humans; Nasopharyngeal Neoplasms; enzymology; genetics; pathology; Promoter Regions, Genetic; genetics; Telomerase; genetics; Thymidine Kinase; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2010;30(4):695-699
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the targeted killing effect of human telomerase reverse transcriptase promoter (hTERTp)/tk gene on human nasopharyngeal carcinoma (NPC) cells.
METHODSThe recombinant plasmid hTERTp/tk/pGL3 was transfected into human NPC HNE1 cells and the expressions of TK and telomerase were investigated. The targeted killing effect induced by hTERTp/tk on HNE1 cells was assessed using RT-PCR and MTT assay.
RESULTSTK gene expression was detected in HNE1 cells transfected by hTERTp/tk/pGL3, and the cells showed reduced telomerase and hTERT expression as compared with the control cells. hTERTp/tk/pGL3 resulted in target killing of HNE1 cells but not of the normal control cells. The tumor cell-killing effect of hTERTp/tk/pGL3 was slightly milder than that of the positive control CMV/tk/pGL3 that produced nonselective cell killing.
CONCLUSIONhTERTp/tk, a tumor-specific expression system, allows targeted tumor cell killing and reduces the activity of telomerase in NPC cells in vitro.
