Effects of human tissue kallikrein gene transfer on the migration of vascular smooth muscule cells.
- Author:
Hui-zhen YU
1
;
Liang-di XIE
;
Peng-li ZHU
;
Chang-sheng XU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Animals; Aorta, Thoracic; cytology; Cell Movement; drug effects; genetics; Cells, Cultured; Gene Transfer Techniques; Humans; Hypertension; pathology; Male; Muscle, Smooth, Vascular; cytology; Platelet-Derived Growth Factor; pharmacology; Proto-Oncogene Proteins c-sis; Rats; Rats, Inbred SHR; Recombinant Proteins; biosynthesis; genetics; pharmacology; Tissue Kallikreins; biosynthesis; genetics
- From: Journal of Southern Medical University 2010;30(4):746-749
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of adenovirus-mediated human tissue kallikrein (Ad-hKLK1) gene transfer on platelet-derived growth factor-BB (PDGF-BB)-induced migration of vascular smooth muscle cells from spontaneously hypertensive rats (VSMC(SHR)).
METHODSA bicistronic recombinant adenovirus vector (Ad-hKLK1) carrying the target hKLK1 gene and the reporter gene EGFP was constructed. VSMCs isolated from the thoracic aorta of male SHR were passaged, and the quiescent VSMC(SHR) in passages 3-6 seeded in 6-well plates were treated with Ad-hKLK1 and control virus. Human PDGF-BB or icatibant Hoe140, a BK B2 antagonistat, was used as the chemoattractant and placed in the bottom chamber of the Boyden chamber. The mRNA expressions of bradykinin B1 receptor and B2 receptor were detected by RT-PCR in VSMC(SHR).
RESULTShKLK1 gene transfer significantly inhibited PDGF-BB-induced migration of VSMC(SHR), with the peak inhibition rate of 34.6% (P<0.001). PDGF-BB significantly increased the mRNA expression of B2 receptor but not B1 receptor in VSMC(SHR).
CONCLUSIONShKLK1 gene transfer can inhibit the migration of VSMC(SHR) induced by PDGF-BB, and the inhibitory effects may be not mediated by bradykinin B2 receptor.
