miR-126 modulates the expression of epidermal growth factor-like domain 7 in human umbilical vein endothelial cells in vitro.
- Author:
Yan-qin SUN
1
;
Fan ZHANG
;
Yi-feng BAI
;
Lin-lang GUO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Down-Regulation; genetics; Endothelial Cells; metabolism; Endothelial Growth Factors; genetics; metabolism; Humans; MicroRNAs; genetics; RNA, Messenger; genetics; metabolism; Transfection; Umbilical Veins; cytology
- From: Journal of Southern Medical University 2010;30(4):767-770
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory effect of miR-126 on epidermal growth factor-like domain 7 (EGFL7) in ECV-304 cells.
METHODSThe miR-126-expressing plasmid targeting EGFL7 (plegfp-N1-miR-126) was constructed and transiently transfected into ECV-304 cells via liposome. The changes in the mRNA and protein expressions of EGFL7 in the transfected cells were analyzed by fluorescence quantitative RT-PCR and Western blotting.
RESULTSTransfection with the recombinant plasmid plegfp-N1/miR-126 resulted in decreased EGFL7 expression with the passage of time, and the expression reached the lowest level at 48 h after the transfection. The expression of EGFL7 protein was reduced by 67% following the transfection in comparison with the control level, while the transfection with the empty vector resulted in a reduction only by 6.5% relative to the control level.
CONCLUSIONSmiR-126 can downregulate EGFL7 expression at the protein level in ECV-304 cells.
