Generation of Dendritic Cells from Human Cord Blood CD34(+) Cells and Their Activation of T Cell-Dependent Antitumor Immune Response In Vitro
- Author:
Guan-Jun WANG
1
,
2
;
Li-Hua XIE
;
Xiao WANG
;
Kai FENG
;
Liang LI
;
Xue-Tao PEI
Author Information
1. Department of Hematology and Oncology, The First Clinical Hospital, Jilin University, Changchun 130021, China
2. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2001;9(2):188-190
- CountryChina
- Language:Chinese
-
Abstract:
To induce the growth and differentiation of dendritic cells (DCs) from human cord blood, CD34(+) cells isolated from human cord blood by mini-MACS were cultured in a liquid culture system with rhSCF, rhGM-CSF, rhTNF-alpha and rhFL for 10 days. Then the induced cells were characterized by DC's morphological and phenotypic properties. In addition, they stimulated the proliferation of allogeneic T cells and possessed an efficient capacity for initiating T cell-dependent antitumor immune responses in vitro. It is concluded that mature DCs could be obtained from human cord blood CD34(+) cells.
