The Application of RACE Technique to Clone the Full-Length cDNA of A Novel Leukemia Associated Gene LRP16

Author: Wei-Dong HAN ¹ ; Li YU ; Fang-Ding LOU ; Quan-Shun WANG ; Yu ZHAO ; Zi-Jiang SHI ; Hai-Jie JIN
Author Information

1. Department of Hematology, The Genral Hospital of PLA, Beijing 100853, China.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2001;9(1):18-21
CountryChina
Language:Chinese
Abstract: LRP16 is a novel gene which was found in our laboratory by using methylation-sensitive restriction landmark genomic scanning (RLGS) technique. In order to clone the full-length cDNA of this leukemia relapse associated gene, the method of rapid amplification of cDNA end (RACE) was employed. By optimizing some procedures of RACE method, the 5'- and 3'-untranslated region of LRP16 cDNA was successfully sequenced. Then, the full length of LRP16 cDNA and open reading frame (ORF) was constructed and was registered in GenBank. The above-mentioned procedure demonstrated RACE technique is a rapid and sensitive method for cloning unknown gene. Especially, it is very useful to cloning the 5'- and 3'-untranslated region of a novel gene.