Establishment of a Human Umbilical Vein Endothelial Cell Line Supporting Hematopoiesis In Vitro
- Author:
Hong-Feng YUAN
1
;
Zi-Kuan GUO
;
Xiao-Dan LIU
;
Shuang-Xi ZHANG
;
Ning MAO
Author Information
1. Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2001;9(1):42-47
- CountryChina
- Language:Chinese
-
Abstract:
Endothelial cells are the critical cell component of hematopoietic microenvironment. For the purpose of facilitating the study on modulating effect of endothelial cells in hematopoiesis, a human umbilical vein cell line, IEC, was established. Primary human umbilical vein endothelial cells were transfected with the plasmid pSV(3neo) carried SV40 large T antigen by lipofection. The IEC cells expressed factor VIII and the UEA I-binding ratio was about 97%. The chromosome variation was existed in the cell line, with the karyotype 45, XX, -18, 18q(+). The cell line retained the proliferative capacity at least 25 passages. IEC cells stimulated the growth of granulocyte-macrophage progenitor cells in coculture of cord blood CD34(+) cells with IEC cells. It is concluded that IEC cell line possessed the biological features of endothelial cell and supported hematopoiesis in vitro.
